Identification of the Efflux Transporter of Ciprofloxacin in Murine Macrophages: Studies with Ciprofloxacin-Resistant Cells
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چکیده
Previous work of our laboratory showed that ciprofloxacin (CIP) is substrate for an ABC transporter belonging to the MRP (Multidrug Resistance-related Proteins) family in murine J774 macrophages [1]. Upon chronical exposure of wild-type (WT) cells to increasing CIP concentrations, we were able to select for CIP-resistant macrophages (RS), which accumulate CIP about 4-fold less than WT cells [2]. MATERIALS & METHODS
منابع مشابه
Identification of the efflux transporter of the fluoroquinolone antibiotic ciprofloxacin in murine macrophages: studies with ciprofloxacin-resistant cells.
Ciprofloxacin, the most widely used totally synthetic antibiotic, is subject to active efflux mediated by a MRP-like transporter in wild-type murine J774 macrophages. To identify the transporter among the seven potential Mrps, we used cells made resistant to ciprofloxacin obtained by long-term exposure to increasing drug concentrations (these cells show less ciprofloxacin accumulation and provi...
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تاریخ انتشار 2008